Axons, neurofibrillary tangles and senile plaques: black
Background : yellow to brown

Elastic fibers:  Blue-black to black
Nuclei: Blue to black
Collagen: Red
Other tissue elements:  Yellow

Acid fast bacilli:   Red.
Nuclei:      Blue.
Other tissue constituents:   Blue.

Acidic Mucins:       Blue.
Neutral Mucins:     Magenta.
Mixtures of above:  Blue/purple
Nuclei:    Deep blue.

Iron (ferric form): Bright Blue
Nuclei: Red
Cytoplasm: Pink

​

Strongly acidic sulfated mucosubstances: Blue
Nuclei: Pink to Red
Cytoplasm: Pale pink

Positive MPO Reaction

• Neutrophilic granulocytes except blast forms

• Eosinophils

• Monocytes except blast forms

Negative MPO Reaction​

• Basophils (weakly positive)

• Lymphocytic cell series

• ​Erythrocyte cell series

Nuclei:  Blue
Collagen: Pale pink
Muscle: Deep Pink
Acidophilic: cytoplasm Red
Basophilic: cytoplasm Purple
Erythrocytes: Cherry red

Amyloid, Elastic Tissue, and Eosinophil Granules: Red

Nuclei: Blue

Erythrocytes: Light pink to brown
Lymphocytes: Blue with purple nucleus
Eosinophil: Blue violet
Eosinophilic Granules: Deep red
Neutrophil: Deep blue to blue-violet
Neutrophil granules: Red
Basophil: Blue violet