RT PCR Kits

White Spot Syndrome Virus (WSSV) is a highly lethal and contagious pathogen of penaeid shrimp. It can cause rapid and complete mortality in shrimp farms, often within a few days of outbreak.

This kit enables detection of WSSV from infected prawn samples via Real-Time Polymerase Chain Reaction (RT-PCR). The targeted region of the WSSV genome is amplified, and detection is based on real-time fluorescence monitoring. A higher initial target copy number results in earlier fluorescence signal detection.

Enterocytozoon hepatopenaei (EHP) is an emerging microsporidian pathogen affecting cultured Penaeus vannamei shrimp across several Southeast Asian countries, including China, Vietnam, Thailand, Indonesia, India, and Malaysia.

This kit is designed for the detection of EHP in infected prawn samples using Real-Time Polymerase Chain Reaction (RT-PCR). It amplifies a specific target region of the EHP genome, and detection is based on fluorescence signal accumulation. The earlier the fluorescence increase is observed, the higher the initial copy number of the nucleic acid target.

Vibrio parahaemolyticus is the causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND), also known as Early Mortality Syndrome (EMS), in shrimp. Additionally, consumption of food contaminated with V. parahaemolyticus can lead to acute gastroenteritis in humans.

This kit is intended for the detection of V. parahaemolyticus in prawn samples using Real-Time PCR (RT-PCR). It targets specific genetic markers of the pathogen, with fluorescence-based detection indicating the presence of the bacteria. Earlier fluorescence signals correlate with higher initial bacterial loads.

Escherichia coli is a common bacterium found in the human gut. While generally harmless, some pathogenic strains can cause severe diarrheal diseases. The presence of E. coli in food or water is a key indicator of fecal contamination and microbiological safety.

This kit enables rapid and specific detection of E. coli in food samples via RT-PCR, following enrichment based on BAM (Bacteriological Analytical Manual) guidelines. The assay includes both internal and positive controls to ensure result accuracy.

For Research Use Only (RUO).

Salmonella species, while commonly present in the human gut, include pathogenic strains that can cause severe foodborne illnesses. Detection of Salmonella is essential for ensuring the microbiological safety of food and water.

This RT-PCR kit is designed for rapid and sensitive detection of Salmonella in food samples, using an enrichment process based on US FDA protocols. It includes internal and positive controls for reliability.

For Research Use Only (RUO).